三、主持或参加的研究项目： (1) 国家自然科学基金项目，家蚕长链非编码RNA参与调控脂肪体发育和细胞自噬的机制研究，32070503，2021-2024，主持，在研 (2) 国家自然科学基金项目，家蚕神经系统piRNA的系统挖掘及功能解析，31470114，2015-2016，主持，已结项 (3) 国家自然科学基金项目，调控家蚕发育非编码RNA（non-coding RNA，ncRNA）的功能解析，31172158，2012-2015，主持，已结项 (4) 国家自然科学基金项目，家蚕嗅觉受体与气味结合蛋白或信息素结合蛋白在嗅觉发生中的功能的研究，30700070，2008-2010，主持，已结项 (5) 河南省高校科技创新人才支持计划，玉米非编码RNA的系统挖掘与功能解析，2010HASTIT011，2010-2011，主持，已结项 (6) 河南省重点实验室专项项目：调控家蚕发育非编码RNA（non-coding RNA，ncRNA）分离及功能解析（102300413209）（已结项） (7) 河南省科技厅国际合作项目：黑腹果蝇气味结合蛋白的表达模式 （已结项） (8) 河南省科技厅自然基金：病原菌诱导下芝麻抗病相关基因的分离（0424070102）（已结项） (9) 省教育厅国际合作项目：按蚊气味结合蛋白的分子分析（0646620017）（已结项） 四、代表性研究成果、授权专利和学术奖励情况： 代表论著： 1.Huili Qiao, Jingya Wang, Yuanzhuo Wang, Juanjuna Yang, Bofan Wei, Miaomiao Li, Bo Wang, Xiaozhe Li, Yang Cao, Ling Tian, Dandan Li, Lunguang Yao, Yunchao Kan. Transcriptome analysis reveals potential function of long non-coding RNAs in 20-hydroxyecdysone regulated autophagy in Bombyx mori. BMC Genomics. 2021;22(1):374. 2.Dandan Li, Huili Qiao, Wujie Qiu, Xin Xu, Tiemei Liu, Qianling Jiang, Renyi Liu, Zhujin Jiao, Kun Zhang, Lijun Bi, Runsheng Chen, Yunchao Kan. Identification and functional characterization of intermediate-size noncoding RNAs in maize. BMC Genomics, 2018, 19(1):730. 3.Hongfei Shi, Yun Hu, Chaoling Leng, Hongling Shi, Zhujin Jiao, Xiaoying Chen, Yunhan Peng, Huan Yang, Yunchao Kan, Lunguang Yao. Molecular investigation of "Candidatus Mycoplasma haemobos" in goats and sheep in central China. Transbound Emerg Dis, 2018, doi: 10.1111/tbed.13021. [Epub ahead of print] 4.Hongfei Shi, Chaoling Leng, Qian Xu, Zhujin Jiao, Hongling Shi, Shiyu Sun, Reng Qiu, Yunchao Kan, Lunguang Yao. Experimental infection of BALB/c mice with a caprine Pestivirus H isolate. Vet Microbiol, 2018, 221:1-7. 5.Cunduo Tang, Hongling Shi, Jianhe Xu, Zhujin Jiao, Fei Liu, Pengju Ding, Hongfei Shi, Lunguang Yao, Yunchao Kan. Biosynthesis of Phenylglyoxylic Acid by LhDMDH, a Novel D-Mandelate Dehydrogenase with High Catalytic Activity. Journal of Agricultural and Food Chemistry, 2018, 66:2805-2811. 6.Cunduo Tang, Honglin Shi,Qinghai Tang, Junzhi Zhou, Lunguang Yao, Jiaozhu Jing, Yunchao Kan. Genome mining and motif truncation of glycoside hydrolase family 5 endo-β-1,4-mannanase encoded by Aspergillus oryzae RIB40 for potential konjac flour hydrolysis or feed additive. Enzyme and Microbial Technology, 2016,(93): 99-104. 7.Dandan Li, Zongcai Liu, Lei Gao, Lifang Wang, Meijuan Gao, Zhujin Jiao, Huili Qiao, Jianwei Yang, Min Chen, Lunguang Yao, Renyi Liu, Yunchao Kan. Genome-Wide Identification and Characterization of microRNAs in Developing Grains of Zea mays L. PloS One. 2016, 11(4): e0153168. 8.Reng Qiu, Yunchao Kan, Dandan Li. Ferritin from the Pacific abalone Haliotis discus hannai: Analysis of cDNA sequence, expression, and activity. Fish & shellfish immunology, 2016, 49: 315-323. 9.Hongfei Shi, Yunchao Kan, Lunguang Yao, liang Leng, Qinghai Tang, Jun Ji, Shiyu Sun. Identification of natural infections in sheep/goats with HoBi-like pestiviruses in China. Transboundary and Emerging Diseases. 2016, 63(5): 480-484. 10.Jianping Duan, Xianxin Meng, Sanyuan Ma, Feng Wang, Huozhen Guo, Liying Zhang, Ping Zhao, Yunchao Kan, Lunguang Yao, Qingyou Xia. The C-terminus of DSXF5 protein acts as a novel regulatory domain in Bombyx mori. Transgenic Res, 2016, 25(4): 491-497. 11.Dandan Li, Zongcai Liu, Lei Huang, Qianling Jiang, Kun Zhang, Huili Qiao, Zhujin Jiao, Lunguang Yao, Renyi Liu, and Yunchao Kan. The expression analysis of silk gland-enriched intermediate-size non-coding RNAs in silkworm Bombyx mori. Insect Science. 2014 Aug;21(4):429-38. 12.Huili Qiao, PeiYuan Deng, DanDan Li, Min Chen, ZhuJin Jiao, ZongCai Liu, YanZhou Zhang, YunChao Kan. Expression analysis and binding experiments of chemosensory proteins indicate multiple roles in Bombyx mori. J Insect Physiol., 2013, 59(7): 667-675. 13.Weiqiang Qian, Daisuke Miki, Heng Zhang, Yunhua Liu, Xi Zhang, Kai Tang, Yunchao Kan, Honggui La, Xiaojie Li, Shaofang Li, Xiaohong Zhu, Xiaobing Shi, Kangling Zhang, Olga Pontes, Xuemei Chen, Renyi Liu, Zhizhong Gong, Jian-kang Zhu. A histone acetyltransferase regulates active DNA demethylation in Arabidopsis. Science, 2012, 336 (6087): 1445-1448. 14.Dandan Li, Yanhong Wang, Kun Zhang, Zhujin Jiao, Xiaopeng Zhu, Geir Skogerboe, Xiangqian Guo, Viswanathan Chinnusamy, Lijun Bi, Yongping Huang, Shuanglin Dong, Runsheng Chen, Yunchao Kan. Experimental RNomics and genomic comparative analysis reveal a large group of species-specific small non-message RNAs in the silkworm Bombyx mori, Nucleic Acids Research, 2011, 39(9):3792-3805. 15.Hongxiang Guo, Yunchao Kan, Weiqun Liu. Differential Expression of miRNAs in Response to Topping in Flue-Cured Tobacco (Nicotiana tabacum) Roots, Plos one, 2011, 6(12) : e28565. 16.Yong Zhang, Xue Zhou, Xie Ge, Jianhao Jiang, Muwang Li, Shihai Jia, Xiaonan Yang, Yunchao Kan, Xuexia Miao, Guoping Zhao, Fei Li, Yongping Huang. Insect-specific microRNA involved in the Development of the Silkworm Bombyx mori. Plos one, 2009, 4(3): e4677. 17.Sha Li, Jean-François Picimbon, Shidong Ji, Yunchao Kan, Chuanling Qiao, Jing-Jiang Zhou and Paolo Pelosi. Multiple functions of an odorant-binding protein in the mosquito Aedes aegypti. BBRC, 2008 (372)464-468. 18.Huili Qiao, Yuanyuan Chen, Zhenzhong Wen, Lijun Bi, Yunchao Kan. Cloning, High Expression of Single-Stranded DNA-Binding Protein and Its Interaction with ssDNA. China Biotechnology. 2007, 27(4):12-17. 19.Huili Qiao, Yuanyuan Chen, Zhenzhong Wen, Lijun Bi, Yunchao Kan. Kinetic Assay and Visualization of Interaction of Single-Stranded DNA-Binding Protein with its Substrate. Acta Biophysica Sinica. 2007, 23(2):145-150. 20.Yunchao Kan, Yongfang Wan, Rowan Mitchell, Frederic Beaudoin, David Leader, Keith Edwards, Rebecca Poole, Daowen Wang and Peter Shewry. Transcriptome analysis reveals differentially expressed storage protein transcripts in seeds of Aegilops and wheat. Journal of Cereal Science.2006, (44):75–85. 21.Jingjiang Zhou, Yunchao Kan, John Antoniw, John Pickett, Lin Field. Genome and EST Analyses and Expression of a Gene Family with Putative Functions in Insect Chemoreception. Chem Senses. 2006, 31(5):453-465. 22.Zejian Guo, Yunchao Kan, Xujun Chen, Debao Li, Daowen Wang. Characterization of a rice WRKY gene whose expression is increased upon pathogen attack and mechanical wounding. Acta Botanica Sinica.2004, 46 (8): 955-964. 23.Yunchao Kan, Shiwang Liu, Zejian Guo, Debao Li. Characterization of a Cyclophilin cDNA from Soybean Cells. Acta Botanica Sinica.2002, 44(2):173-176. |