CURRICULUM VITAE
Zixing Dong
Ph.D., Lecturer,
Nanyang Normal University
No. 1638 Wolong Road
Nanyang City, Henan Province, P. R. China. 473061
Email: dzx@nynu.edu.cn
Education
2008.09-2014.01 Ph.D., Fermentation Engineering, School of Biotechnology, Jiangnan University, China
2004.09-2008.07 B.S., Bioengineering, College of Life Sciences, Northeast Agricultural University, China
Work Experience
2024.05-present Lecturer, College of Life Sciences, Nanyang Normal University
2020.09-2024.04 Lecturer, College of Life Sciences and Agricultural Engineering, Nanyang Normal University
2019.04-2020.08 Lecturer, College of Agricultural Engineering, Nanyang Normal University
2015.06-2019.03 Research assistant, College of Chemical Engineering and Materials Science, Tianjin University of Science & Technology
2014.11-2015.05 Research assistant, College of Biotechnology, Tianjin University of Science & Technology
Courses
Proteomics, principles of fermentation technology, professional English for biology students and enzyme engineering for undergraduate students
Research Interests
(1) Enzyme engineering and biocatalysis;
(2) Discovery and modification of novel biocatalysts, and their applications in organic synthesis
Funding
2022.01-2023.12 Elucidation of the substrate recognition mechanism of bile salt hydrolase LpBBE7_BSH from Lactobacillus plantarum, funded by Natural Science Foundation of Henan Province in China, Grant No. 222300420251, PI
2021.01-2024.12 Construction and high-efficient expression of acid- and thermotolerant isoamylase for the saccharification of starch, funded by Nanyang Normal University, Grant No. 2020ZX004, PI
2019.01-2021.12 Enzyme library, enzymes cocktail and new strains for integrated bioprocessing of agricultural biomass, funded by Ministry of Science and Technology of the People's Republic of China, Grant No. YS2018YFGH000002, participate
2019.01-2022.12 Detailed profiling of the dynamic proteolysis process and site specificity of food alkaline proteases by terminomics, funded by National Natural Science Foundation of China, Grant No. 31871784, participate
2018.01-2021.12 Relationship between the structure and function of cholesterol 7- dehydrogenases for the biosynthesis of vitamin D3 precursors, funded by National Natural Science Foundation of China, Grant No. 31771911, participate
2018.01-2020.12 Innovative techniques for enzymatic and bio-catalysis, funded by Tianjin Municipal Commission of Education, Grant No. TD12-5002, participated
2014.10-2015.09 Screening and optimization the fermentation conditions of thermostable lipase-producing microorganisms, funded by Foundation of Tianjin Municipal Science and Technology Commission, Grant No. 14RCGHSY00181, participate
Selected Publications
1. Xinxin Liu, Yao Wang, Jianhui Zhang, Yunfeng Lu, Zixing Dong, Chao Yue, Xianqing Huang, Sipu Zhang, Dandan Li*, Lunguang Yao*, Cunduo Tang*. Engineering Escherichia coli for high-yielding 2,5-dimethylpyrazine synthesis from L-threonine by reconstructing metabolic pathways and enhancing cofactors regeneration[J]. Biotechnol Biofuels Bioprod, 2024, 17(1): 44.
2. Xiang Zhang, Yali Xiao, Xin Shi, Hongling Shi, Zixing Dong, Cunduo Tang*. The role of cellular senescence-related genes in Asthma: Insights from bioinformatics and animal experiments. Int Immunopharmacol, 2024, 130, 111770.
3. Zixing Dong*, Shuangshuang Yang, Kun Zhang, Cunduo Tang, Yunchao Kan, Lunguang Yao*. Biochemical characterization of a novel alkaline-tolerant Xaa-Pro dipeptidase from Aspergillus phoenicis[J]. Fermentation, 2023, 9 (11): 978.
4. Zixing Dong*, Shuangshuang Yang, Zhengtian Zhang, Cunduo Tang, Yunchao Kan, Lunguang Yao*. Prolyl aminopeptidases: Reclassification, properties, production and industrial applications[J]. Process Biochem, 2022, 118: 121-132.
5. Zixing Dong*, Shuangshuang Yang, Xiaoxiao Dong, Yongna Yang, Xueting Yan, Jiejie Su, Cunduo Tang, Lunguang Yao, Yunchao Kan. Characteristics, protein engineering, heterologous production and industrial applications of microbial isoamylases. Starch - Stärke, 2022, 74(1-2): 2100192.
6. Zixing Dong*, Shuangshuang Yang, Byong H. Lee. Bioinformatic mapping of a more precise Aspergillus niger degradome[J]. Sci Rep, 2021, 11(1): 693.
7. Xiaozhu Tian, Rong Li, Yiming Jiang, Fei Zhao, Zhengsheng Yu, Yiqing Wang, Zixing Dong, Pu Liu*, Xiangkai Li*. Bifidobacterium breve ATCC15700 pretreatment prevents alcoholic liver disease through modulating gut microbiota in mice exposed to chronic alcohol intake[J]. J Funct Foods, 2020, 72: 104045.
8. Cunduo Tang, Xiang Li, Hongling Shi, Yuanyuan Jia, Zixing Dong, Zhujin Jiao, Linfeng Wang, Jianhe Xu, Lunguang Yao*, Yunchao Kan*. Efficient expression of novel glutamate decarboxylases and high level production of γ-aminobutyric acid catalyzed by engineered Escherichia coli. Int J Biol Macromol. 2020, 160: 372-379.
9. Zixing Dong*, Cunduo Tang, Yunfeng Lu, Lunguang Yao, Yunchao Kan. Microbial oligo-α-1,6-glucosidase: Current developments and future perspectives [J]. Starch - Stärke, 2020, 72 (1-2): 1900172.
Patents
1. Cunduo Tang, Lunguang Yao, YunChao Kan, Hongling Shi, Zixing Dong, et al. A gene encoding glutamate decarboxylase LsGAD, a recombinant bacterium and a kombucha fermentation bacterium, China National invention patent, ZL201911234346.7
2. Lunguang Yao, Yunchao Kan, Cunduo Tang, Hongling Shi, Zixing Dong, et al. A gene encoding glutamate decarboxylase, a recombinant bacterium and use thereof, China National invention patent, ZL201911234348.6
3. Jian Chen, Juan Zhang, Zixing Dong, et al. A mutant of bile salt hydrolase and use thereof, China National invention patent, ZL201310557568.9
4. Jian Chen, Zixing Dong, Juan Zhang, et al. A bile salt hydrolase-producing lactic acid bacterium, and its screening method and use thereof, China National invention patent, ZL 201110119378.X
5. Jian Chen, Juan Zhang, Zixing Dong, et al. A recombinant bacterium with bile salt hydrolase activity, and its construction method and use thereof, China National invention patent, ZL 201110119810.5
6. Jian Chen, Qian Zhang, Guocheng Du, Juan Zhang, Zixing Dong, et al. A strain of Lactobacillus paracasei and use thereof, China National invention patent, ZL 201010238658.8
Awards and Honors
2024 “Challenge Cup” National College Student Business Plan Competition, Second prize
2022 The 8th Henan Provincial College Students “Internet+” Innovation and Entrepreneurship Competition, Third prize