姓 名：Camilo Ayra-Pardo

性 别：male

职 称：Associate Professor, PhD

所在系别：Henan Provincial Engineering Laboratory of Insect Bio-reactor

电子邮箱：cayrapardo73@yahoo.com
 

个人简历：

2017.10—present date：Associated Professor, Nanyang Normal University, Nanyang, Henan, China

•2017.01-2017-07: Invited Professor at School of Biology, University of Havana, Cuba

•2015.01-2016.12: Senior (Full) researcher, Centre for Genetic Engineering and Biotechnology (CIGB), Havana, Cuba

•2014.09-2014.12: Swiss NSF fellow at Department of Plant Molecular Biology, University of Lausanne, Switzerland

•2010.07-2014.08: Senior (Full) researcher, Centre for Genetic Engineering and Biotechnology (CIGB), Havana, Cuba

•2010.02-2010.06: Invited staff member at Department of Life Sciences, Imperial College London, UK

•2007.08-2010.01: Associated researcher, Centre for Genetic Engineering and Biotechnology (CIGB), Havana, Cuba

•2007.03-2007.07: UK Royal Society fellow at Department of Life Sciences, Imperial College London, UK

•2004.09-2008.12: Ph.D. in Biological Sciences, University of Havana, CUBA

•2004.02-2004.08: UK Royal Society fellow at Department of Biochemistry, University of Cambridge, UK

•1999.03-2004.01: Assistant researcher, Centre for Genetic Engineering and Biotechnology (CIGB), Havana, Cuba

•2000.03-2000.11: Project Management, School of Business of Madrid, SPAIN

•1998.02-1999.02: Assistant researcher, Centre for Research and Advanced Studies of the National Technological Institute (CINVESTAV-IPN), Mexico City, Mexico

•1996.08—1998.01：Junior researcher, Centre for Genetic Engineering and Biotechnology (CIGB), Havana, Cuba

•1991.09—1996.07：B.Sc. major Biochemistry, School of Biology, University of Havana, Cuba
 

教学工作：

From October 2017 – Present date: Professor. School of Agricultural Engineering, Nanyang Normal University, Nanyang, Henan, China. Lectures on diverse topics: Plant Molecular Breeding, Agroecology and Biotechnology, Industrial Biochemistry, Advances in Microbiology

•September 2009- July 2015. Invited Professor. School of Biology, University of Havana, CUBA. Lectures on: Agricultural Biotechnology, Plant Molecular Biology, Molecular Entomology, Molecular Host-Pathogen Interaction

•Invited lecturer in Master programs majoring Biotechnology at: University of Lausanne (SWITZERLAND, October 2014), University of Granada (SPAIN, November 2010), and CINVESTAV-IPN institute (MEXICO, 1998).

研究方向：

Insect pest control, Insect behaviour and microbiome

科研成果：

RESEARCH GRANTS:

1.2018.01–2019.12: Nanyang Normal University (NYNU) Research Grant. China–UK–NYNU–RRES Joint Laboratory for Insect Biology. “Host preference behaviour in cotton bollworm and gut microbiota”. Group of Prof. Yunchao Kan. (¥ 150,000)

2.2014.08: Swiss National Science Foundation (SNSF) International Grant for a research visit to University of Lausanne. “Role of an Arabidopsis thaliana ERF/AP2 transcription factor for plant defence against insect herbivores”. Group of Prof. Philippe Reymond (CHF 12,030.00)

3.2007.03–2010.12: IFS (International Foundation for Sciences at SWEDEN; http://www.ifs.se/) Research Grant # C/3579-2. “Identification, cloning and characterization of Spodoptera frugiperda midgut genes involved in the defensive response against the Cry1Ca toxin of Bacillus thuringiensis”– Principal investigator (105,602.40 SEK)

4.2007.01: UK Royal Society International Grant (2006/R3) for a research visit to Imperial College London. “Identification of insect genes involved in defensive response to Bt Cry toxins”. Group of Prof. Denis J. Wright (£ 5,212.00)

5.2004.01: UK Royal Society International Exchange Grant for a research visit to University of Cambridge. “Protein engineering to improve the potency and specificity of Bacillus thuringiensis insecticidal toxins”. Group of Prof. David J. Ellar (£ 7,111.00)

6.2004.01- 2006.12: IFS Research Grant # C/3579-1. “Molecular identification of Spodoptera frugiperda gut genes induced after short-term exposure to sublethal concentrations of the Cry1Ca toxin from Bacillus thuringiensis bacterium” – Principal investigator (86,400 SEK)

7.2003.01-2017.08: Centre for Genetic Engineering and Biotechnology, Institutional Research grant #3031-243 for Biotech solutions regarding insect pests control in Cuban agriculture – Head of research projects (CUP 5,000,000)

8.1996.09- 1999.01: IFS Research Grant # F/2560-1. “Bacillus thuringiensis spp. biodiversity: molecular screening of the national collection” – Co-applicant (86,400 SEK)

PEER-REVIEWED PUBLICATIONS IN SCI JOURNALS

1.R Portieles, ME Ochagavia, E Canales, Y Silva, O Chacón, I Hernández, Y López, M Rodríguez, R Terauchi, C Borroto, R Santos, MD Bolton, C Ayra-Pardo, et al. High-throughput SuperSAGE for gene expression analysis of Nicotiana tabacum–Rhizoctonia solani interaction. BMC Res. Notes 2017; 10: 603. I.F: 0.669

2.C Ayra-Pardo*, ME Ochagavia, B Raymond, A Gulzar, L Rodríguez, et al. HT-SuperSAGE of the gut tissue of a Vip3Aa-resistant tobacco budworm strain provides insights into the basis of resistance. Insect Sci. 2017; DOI: 10.1111/1744-7917.12535 (*corresponding author) I.F.: 2.026

3.E Canales, Y Coll, I Hernández, R Portieles, M Rodríguez, Y López, M Aranguren, E Alonso, R Delgado, M Luis, L Batista, C Paredes, M Rodríguez, M Pujol, ME Ochagavia, V Falcón, R Terauchi, H Matsumura, C Ayra-Pardo et al. Candidatus Liberibacter asiaticus, causal agent of citrus Huanglongbing, is reduced by treatment with brassinosteroids. PLoS One 2016; 11(1): e0146223. I.F.: 2.806

4.C Ayra–Pardo*, B Raymond, A Gulzar, L Rodríguez, I Morán, N Crickmore, DJ Wright. Novel genetic factors involved in resistance to Bacillus thuringiensis in Plutella xylostella. Insect Mol. Biol. 2015; 24(6): 589-600. (*corresponding author) I.F.: 2.884

5.P Téllez, B Raymond, I Morán, L Rodríguez, DJ Wright, CG Borroto, C Ayra-Pardo*. Strong oviposition preference for Bt over non-Bt maize in Spodoptera frugiperda and its implications for the evolution of resistance. BMC Biology 2014; 12(1): 48. (*corresponding author) I.F.: 6.779

6.R. Portieles, C Ayra-Pardo, E González, A Gallo, R Rodríguez, O Chacón, et al. NmDef02, a novel antimicrobial protein isolated from Nicotiana megalosiphon confers high-level pathogen resistance under greenhouse and field conditions. Plant Biotechnol. J. 2010; 8: 678-690. I.F.: 7.443

7.L Rodríguez, D Trujillo, O Borrás-Hidalgo, D.J. Wright, C Ayra-Pardo*. RNAi-mediated knockdown of a Spodoptera frugiperda trypsin-like serine-protease gene reduces susceptibility to a Bacillus thuringiensis Cry1Ca protoxin. Environ. Microbiol. 2010; 12: 2894–2903. (*corresponding author) I.F.: 5.395

8.L Rodríguez, D Trujillo, O Borrás, DJ. Wright, C Ayra-Pardo*. Molecular characterization of Spodoptera frugiperda – Bacillus thuringiensis Cry1Ca toxin interaction. Toxicon 2008; 51: 681-692. (*corresponding author) I.F: 2.492

9.C Ayra-Pardo*, P Davis, DJ. Ellar. The mutation R423S in the Bacillus thuringiensis hybrid toxin CryAAC slightly increases toxicity for Mamestra brassicae L. J Invertebr. Pathol. 2007; 95: 41-47. (*corresponding author) I.F.: 2.379

10.O Borras, BPHJ Thomma, C Collazo, O Chacón, CJ Borroto, C Ayra-Pardo, et al. EIL2 transcription factor and glutathione synthetase are required for defense of tobacco against tobacco blue mold. Mol. Plant Microbe In. 2006; 19: 399-406. I.F: 4.332

11.A Fuentes, PL Ramos, C Ayra-Pardo, M Rodríguez, N Ramirez, M Pujol. Development of a highly efficient system for assessing recombinant gene expression in plant cell suspensions via Agrobacterium tumefaciens transformation. Biotechnol. Appl. Biochem. 2004; 39: 355-361. I.F.: 1.413

12.P Tellez, C Ayra-Pardo*, D Barberia, E Gonzalez. Insect-resistant transgenic plants: the CIGB experience. Phytopathology 2002; 92(6s): 132-133. (*corresponding author) I.F.: 2.896

13.C Ayra-Pardo*, I.L. Montejo, R.I. Vázquez, C García. Beta-D-glucuronidase gene from Escherichia coli is a functional reporter in the methylotrophic yeast Pichia pastoris. Lett. Appl. Microbiol. 1999; 29: 278-283. (*corresponding author) I.F.: 1.575

14.G.A. de la Riva, J González, R.I. Vázquez, C Ayra-Pardo. Agrobacterium tumefaciens: plant transformation tool without frontiers. Electr. J. Biotechnol. 1998; 3: http://ejb.ucv.cl I.F.: 1.527

15.R.I. Vázquez, A.F. Martínez, C Ayra-Pardo, J González, G.A. de la Riva. Biochemical characterization of the third domain from Cry1A toxins. Biochem. Mol. Biol. Int. 1998; 45(5): 1011-1020. (currently IUBMB life) I.F.: 3.141

16.D Prieto, R.I. Vázquez, C Ayra-Pardo, J González, G.A. de la Riva. Bacillus thuringiensis: from biodiversity to biotechnology. J. Industr. Microbiol. Biotechnol. 1997; 19: 202-219. I.F.: 2.810

PATENTS

1.O. Borrás, R. Portieles, M. Pujol, G. Enriquez, E.M. Gonzalez, C Ayra-Pardo. Aminoacids and nucleic acid sequences for controlling pathogens. WO 2009/117975A1

2.O Borras, R Portieles, M Pujol, G Enriquez, E Gonzalez, C Ayra-Pardo. Nucleic and aminoacid sequences for the control of pathogen agents. US8,450,559B2

3.G.A. de la Riva, R.I. Vázquez, R. Pajón, J. González, A.F. Martínez, C Ayra-Pardo (2004). Use of Cry proteins from Bacillus thuringiensis as immune stimulators agents and as vectors for the presentation of vaccine epitopes. National Publication Number: CU/22977. International Patent Classification: A61K 39/02.

BOOK CHAPTER

1.C Ayra-Pardo, O Borras-Hidalgo (2018). Fall armyworm (FAW; Lepidoptera: Noctuidae): moth oviposition and crop protection. In: Olfactory concepts of insect control. (Jean-François Picimbon, Ed.). Springer (in press).

PEER-REVIEWED PUBLICATIONS IN NON-SCI JOURNALS:

1.M Morejon, JA Herrera, C Ayra-Pardo, et al. Alternatives in nutrition of transgenic maize FR-Bt1 (Zea mays L.): response in growth, development and production. Cultivos Tropicales 2017; 38(4): 146-155. ISSN 1819-4087

2.P Téllez, C Ayra-Pardo*, I Morán, L Rodríguez, AE Sosa, O Oliva, et al. New knowledge on insect-resistance management for transgenic Bt corn. Biotecnol. Apl. 2016; 33: 1511-1513. (*corresponding author) ISSN 1027-2852

3.D Hernández, H Aragón, M González, G González, A González, A Tamayo, I Morán, P Téllez, E Sánchez, M Castillo, W Ferro, C Ayra-Pardo*. Monoclonal antibody generation and characterization for Vip3Aa20 quantification in transgenic corn plants. BioProcessing J. 2016; 15: 30-43. (*corresponding author) ISSN 1538-8786

4.L Rodríguez, I Morán, C Ayra-Pardo*, et al. Functional identification of three new genes involved in resistance to Bacillus thuringiensis in Plutella xylostella. Biotecnol. Apl. 2016; 33: 3501-3503. (*corresponding author) ISSN 1027-2852

5.I Morán, P Téllez, J Aguiar, D Hernández, L Rodríguez, M Ponce, R Valdés, C Ayra–Pardo*. Maize genetic transformation procedure improvement using Agrobacterium tumefaciens and FR-28 Cuban synthetic corn variety as model. J. Agronomy 2014; 13(4): 169-178. (*corresponding author) ISSN 18125379

6.D Hernández, L Rodríguez, R Valdés, I Morán, P Téllez, A Riberon, Y Ramos, L Gomez, C Ayra-Pardo*. Bacillus thuringiensis Vip3Aa1 expression and purification from E. coli to be determined in seeds and leaves of genetically modified corn plants. J. Agronomy 2013; 12(4): 153-167. (*corresponding author) ISSN 18125379

7.L Rodríguez, O Borrás, P Téllez, I Morán, M Ponce, Y Fernández, C Ayra-Pardo*. Identification of a new trypsin from Spodoptera frugiperda involved in a defensive mechanism against the Bacillus thuringiensis Cry1Ca1 toxin. Biotecnol. Apl. 2011; 28(3): 176-179. (*corresponding author) ISSN 1027-2852

8.C Ayra-Pardo*, L Rodríguez, D Trujillo, et al. Broadening the target host range of the insecticidal Cry1Ac1 toxin from Bacillus thuringiensis by biotechnological means. Biotecnol. Apl. 2008; 25(3): 1-3. (*corresponding author) ISSN 1027-2852

9.C Ayra-Pardo*, L Rodríguez, D Trujillo, et al. The mutation R423S in the Bacillus thuringiensis hybrid toxin CryAAC increases in vitro oligomerisation and in vivo toxicity against Spodoptera frugiperda. Biotecnol. Apl. 2007; 24: 126-131. (*corresponding author) ISSN 1027-2852

10.O Borrás, O Chacón, R Portieles, C Collazo, Y López, CJ Borroto, C Ayra-Pardo, et al. Identification of new disease resistance genes in tobacco via functional genomics. Biotecnol. Apl. 2007; 24(1): 76-78. ISSN 1027-2852

11.C Ayra-Pardo*, L Rodríguez-Cabrera, Y Fernández-Parlá, P Téllez-Rodríguez. Increased activity of a hybrid Bt toxin against Spodoptera frugiperda larvae from a maize field in Cuba. Biotecnol. Apl. 2006; 23: 236-239. (*corresponding author) ISSN 1027-2852

12.R Portieles, C Ayra-Pardo, O Borras. Basic insight about plant defensins. Biotecnol. Apl. 2006; 23: 75-78. ISSN 1027-2852

13.M Pujol, CA Hernández, R Armas, Y Coll, JA Rubi, M Pérez, C Ayra-Pardo, et al. Inhibition of Heliothis virescens larva growth in transgenic tobacco plants expressing cowpea trypsin inhibitor. Biotecnol. Apl. 2005; 22: 127-130. ISSN 1027-2852

14.C Ayra-Pardo*. Genetic Modified Organisms and Biodiversity: assessing the threats. Biotecnol. Apl. 2003; 20: 1-8. (*corresponding author) ISSN 1027-2852

15.G Enriquez, A Arencibia, G Selman-Houseim, L Hernandez, K Tiel, A Fuentes, P Tellez, D Munoz, E Carmona, A Salazar, N Soto, L Trujillo, C Menendez, Y Coll, D Alfonso, M Pujol, C Ayra-Pardo. Sugarcane biotechnology and genetic engineering experience at CIGB. Proc. Int. Soc. Sugar Cane Technol. 2001; 24: 578-579.

16.M Saura, C Ayra-Pardo*. Biochemical and Genetic characterization of thirteen Cuban Bacillus thuringiensis isolates. J. Biochem. Mol. Biol. Biophys. 2001; 5: 435-440. (*corresponding author) ISSN 1025-8140

17.J González, RI Vázquez, DL Prieto, C Ayra-Pardo, et al. Genetic transformation of cabbage (Brassica oleraceae var capitata) with Bacillus thuringiensis cry1Ab and cry1B genes. Proc. Interamer. Soc. Trop. Hort. 1998; 42: 367-373.

18.C Ayra-Pardo*, C García, GA de la Riva. A single-step screening procedure for Pichia pastoris clones, by PCR. Biotecnol. Apl. 1998; 15: 175-177. (*corresponding author) ISSN 1027-2852